Table 1.

Effect of ITP on single-molecule assays with loads applied to RNA or DNA

Assay	Force (pN) direction	[ITP] (μM)	Pause density (kb−1) (N)
RNA-pulling	28	0	0.12 ± 0.03 (46)
RNA-pulling	28	200	0.20 ± 0.10 (4)
DNA-pulling	28 assisting	0	0.20 ± 0.10 (24)
DNA-pulling*	8 assisting	0	<0.03 (1)
DNA-pulling*	8 assisting	200	0.15 ± 0.10 (2)
DNA-pulling*	8 hindering	0	0.95 ± 0.21 (56)
DNA-pulling*	8 hindering	200	1.46 ± 0.29 (26)

^*data from (Shaevitz et al., 2003).