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. Author manuscript; available in PMC: 2007 Jan 1.
Published in final edited form as: Neuroscience. 2006 Feb 24;139(3):1017–1029. doi: 10.1016/j.neuroscience.2005.12.058

Figure 1.

Figure 1

(A) Representative gel electrophoreses showing competitive PCR-analysis for BDNF mRNA content in frontal cortex obtained from one sham rat. Decreasing concentrations of standard cRNA (50-3.12 pg) were added to a constant amount (1 μg) of total RNA isolated from frontal cortex. The mixtures were reverse transcribed and PCR-amplified in the presence of trace amounts of [32P]dCPT; aliquots were digested by Bgl II and electrophoresed on 1.5% agarose gel. The higher molecular size band corresponds to the amplification product arising from the mRNA, whereas the lower bands arise from cRNA generated from the internal standard digested by Bgl II. (B) Data derived from the agarose gels are plotted as the counts incorporated into the amplified cRNA standard divided by the counts incorporated into the corresponding mRNA amplification product versus the known amount of internal standard cRNA added to the test sample. The point of equivalence represents the amount of BDNF mRNA.