Skip to main content
. Author manuscript; available in PMC: 2006 Jul 24.
Published in final edited form as: Neurosurgery. 2005 Mar;56(3):590–604. doi: 10.1227/01.NEU.0000154060.14900.8F

FIGURE 5.

FIGURE 5

DFU treatment altered relative eicosanoid levels in the injured cortex. Eicosanoid levels increased in the ipsilateral cortex by 24 hours after injury. DFU (1 mg/kg) initiated 10 minutes before injury decreased PGs and HETEs and resulted in an amplification of EETs, possibly because of arachidonic acid shunting. Fresh-frozen parietal cortex was microdissected from 300-μm sections, and lipids were extracted and derivatized as described in Materials and Methods. PGs, HETEs, EETs, and their dihydroxy metabolites (DiHETEs) were quantified by fluorescence-detected HPLC (109). Individual peak areas (corrected for recovery) were grouped by class and summed, corrected for tissue wet weight, and normalized to the contralateral side. Averages (n = 3 animals per group) shown for sham/vehicle (SHAM, light bars), injured/vehicle (INJURED, striped bars), and injured/DFU (INJ/DFU, 1 mg/kg, dark bars). Note the discontinuity in the y axis, showing the extent of EET increase.

HHS Vulnerability Disclosure