Human tissue expression pattern of Tre2, USP32, and TBC1D3. cDNA panels (CLONTECH) were screened by semiquantitative RT-PCR and are as follows: 1, heart; 2, brain; 3, placenta; 4, lung; 5, liver; 6, skeletal muscle; 7, kidney; 8, pancreas; 9, spleen; 10, thymus; 11, prostate; 12, testis; 13, ovary; 14, small intestine; 15, peripheral blood leukocyte; and 16, negative control. Specific RT-PCR products for Tre2, USP32, and TBC1D3 are designed by amplification across the unique juncture for the respective transcripts. Glyceraldehyde-3-phosphate dehydrogenase (G3PDH) amplification was used as a control.