Figure 2.

Primary sequence alignments of NPC2 orthologs and related proteins. (A) Alignment of mammalian NPC2 proteins. Numbering starts with the first residue after the signal sequence. Conserved residues are highlighted in yellow, with identical residues printed in red. Secondary structure elements of the bNPC2 structure are shown above the primary sequence. Red lines connect cysteine residues that form disulfide bonds. Three potential glycosylation sites are marked by triangles with the absolutely conserved site, which is glycosylated in bNPC2, indicated in red. (B) Alignment of bNPC2 and dust mite allergen proteins Der p 2 and Der f 2. Numbering corresponds to bNPC2 with coloring as in A.