Figure 2.

Expression of GzmB and CTL activity by GzmB genotype. PBMC from untreated long-term asymptomatic HIV⁺ patients enrolled in the ALT cohort were thawed, and GzmB expression in CD8⁺ lymphocytes was measured by intracellular fluorescence-activated cell sorter staining either directly (A and B) or after 4 days culture in the presence of 1 μg/ml PHA-P (C and D). Results are shown as the percentage of CD8⁺ lymphocytes staining positive for GzmB (A and C), and as the number of PE molecules bound per cell in the GzmB-positive population (B and D). Each symbol represents one patient. Anti-HIV CTL activity in cell lines derived from PBMC of HIV⁺ patients enrolled in the ALT cohort was tested against autologous EBV-immortalized B-lymphoma lines infected with recombinant vaccinia viruses expressing HIV gag, pol, env, nef, rev, tat, or vif proteins. For each HIV protein, the results of a ⁵¹Cr release assay were noted as positive or negative, and the number of positive results were scored for each patient (E). Each symbol represents one patient. GzmB genotypes are as follows: 0, QPY/QPY; 1, QPY/RAH; 2, RAH/RAH.