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. 2003 Feb 18;100(5):2568–2573. doi: 10.1073/pnas.0530167100

Figure 1.

Figure 1

Diagram of 23S RNA launching plasmid (A) and launched RNA detected by Northern blots (B–D). (A) pRE637 contains the complete 23S RNA cDNA sequence (bold line) downstream of the PGK1 promoter (PGK1). The antigenomic hepatitis delta virus ribozyme (R), its cleavage site (vertical arrow), p104, and its conserved RDRP motifs (A–D) are shown. The viral genome contains 6 and 59 nt in the 5′ and 3′ untranslated regions, respectively. (B–D) Detection of launched viral RNA in growing (B) or induced (C) cells or in a ski2 strain (D) by Northern blots hybridized with 23S RNA positive or negative strand-specific probes. In B, four independent transformants (1–4) and recipient cells without plasmid (C) are shown. In C, ethidium-bromide staining of the gel is also shown. The positions of 23S RNA, its double-stranded form (T dsRNA), as well as yeast rRNAs and 20S RNA are indicated. For clarity the autoradiogram in D was exposed five times shorter than the one in C with the same probe.