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. 2003 Feb 18;100(5):2568–2573. doi: 10.1073/pnas.0530167100

Figure 2.

Figure 2

Proof of 23S RNA virus generation from the launching plasmid. (A) Diagram of 23S RNA and one of two SmaI sites analyzed by RT-PCR amplification. Two oligonucleotides used for amplification are shown by the arrows. The RT-PCR products (644 nt) from endogenous virus will produce two fragments (394 and 250 bp) after SmaI digestion, whereas those from launched virus are expected to be resistant to the enzyme. (B and C) RT-PCR products from endogenous and launched 23S RNA viruses before (B) and after (C) SmaI treatment. Ethidium-bromide staining of agarose gels is shown. Lambda HindIII markers and the sizes of digested and undigested PCR products are indicated. In the third lane of C (mixture) the PCR products from endogenous and launched virus are mixed together and then digested with the enzyme. Note that in C the amount of the larger fragment (394 bp) appears less than that of the smaller fragment (250 nt) because of comigration of the bromophenol-blue dye.