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. 1999 Jan 19;96(2):355–360. doi: 10.1073/pnas.96.2.355

Figure 1.

Figure 1

(A) Regulatory system. The regulator GLVPc′ consists of a mutated human progesterone receptor–LBDΔ, a DNA binding domain of yeast GAL4 (GAL4–DNA binding domain), and an activation domain of herpes simplex virus (VP-16). Regulator GLp65 contains the activation domain of p65. The target consists of four GAL4 binding sites and a TATA-box linked to the luciferase reporter gene. Luc, luciferase; PR, progesterone receptor. (B) Mifepristone-dependent target gene induction by GLVP compared with GLp65. GLVPc′ or GLp65 (0.3 μg per well on a 6-well plate) were transiently transfected in Hela cells with the 17 × 4-TATA-luciferase as a reporter (0.3 μg per well on a 6-well plate) (B) by using 17 × 4-tk-luciferase as a reporter (C). The luciferase activity is shown as relative luciferase units (RLU). Control, transfection of the reporter and expression vector backbone; +, presence of mifepristone (Ru 486) [10−8]; −, absence of mifepristone (Ru 486). Error bars show standard deviation.