Figure 3.
Induction of hGH on adenoviral transduction. (A) C57BL/6 mice (8–10 weeks old) were infected in the tail vein at day 0 with 1 × 109 infectious particle units of hGH-GLp65. Mifepristone (Ru 486) (250 μg/kg) was administered every second day after infection for a period of 2 weeks as indicated by arrows. Mice were bled at different time points, and serum hGH was analyzed by radioimmunoassay. Mice 1 and 2 received i.p. injections of mifepristone; mouse 3 (minus mifepristone) received sesame oil. hGH serum levels are shown micrograms per milliliter. (B) Kinetics of inducing hGH in mice 2 weeks after adenoviral infection. Mice infected for 2 weeks with the regulable adenoviral construct hGH-GLp65 were induced with 500 μg/kg mifepristone as indicated by an arrow. Blood was drawn from the mice 3, 6, 12, 24, 48, 72, 120, and 192 h after mifepristone (Ru 486) administration, and hGH was measured in the serum by a radioimmunoassay. hGH serum levels of individual mice are shown in micrograms per milliliter.