Table 1.
Adenovirus-mediated inducible hGH expression in hepatocytes
| Construct | Mifepristone | hGH, ng/ml |
|---|---|---|
| No virus | − | n.d. |
| No virus | + | n.d. |
| hGH-GLp65 | − | 5 |
| hGH-GLp65 | + | 19,000 |
| hGH-H-GLp65 | − | n.d. |
| hGH-H-GLp65 | + | 22,000 |
The viral construct without insulator (hGH-GLp65) was compared with the construct containing the insulator (hGH-H-GLp65). Cells 2 × 105 were infected with 1 × 109 viral particles. Three hours after infection, the media was changed, and antiprogestin mifepristone at a concentration of 10−8 was added. Twenty-four hours later, hGH was monitored by using a radioimmunoassay. The table displays the amounts of hGH in nanograms per milliliter cell media. n.d., no detectable levels of hGH.