Figure 3.

Confocal micrograph showing immunohistologic staining of the Icon in the CNV of the pig. Choroidal flat mounts were prepared 1 week after diode laser photocoagulation in the pig, incubated with Icon protein, and stained with a FITC-labeled antibody against the Fc domain of the Icon. The endothelial cells of the CNV membrane (arrowhead) are stained green (speckled pattern), whereas the normal retinal vascular endothelium is not stained (arrow). The normal choroidal endothelium also did not stain (not shown) (×2,000).