Table 1.
Selected proteins differentially expressed in P. aeruginosa during growth in low magnesium
| Gene no. | Protein | n | u-ICAT | Fold abundance | SD |
|---|---|---|---|---|---|
| Conserved magnesium stress response | |||||
| 1179 | Two-component response regulator PhoP | 6 | 1 | 10.34 | 0.96 |
| 3552 | Conserved hypothetical protein, PmrH homologue | 7 | 1 | 2.84 | 0.25 |
| 3553 | Probable glycosyl transferase, PmrF homologue | 1 | 1 | 2.33 | na |
| 3554 | Conserved hypothetical protein, PmrI homologue | 34 | 7 | 6.09 | 1.16 |
| 4635 | Conserved hypothetical protein, MgtC homologue | 13 | 1 | 3.99 | 0.56 |
| 4825 | Magnesium transport ATPase MgtA | 85 | 3 | 5.80 | 1.75 |
| Quorum sensing and adaptation | |||||
| 0934 | GTP pyrophosphokinase RelA | 2 | 1 | 1.53 | 0.00 |
| 0996 | Probable coenzyme A ligase | 1 | 1 | 1.57 | na |
| 0997 | Hypothetical protein | 16 | 2 | 1.57 | 0.26 |
| 0998 | Hypothetical protein | 6 | 2 | 2.04 | 0.24 |
| 0999 | 3-Oxoacyl-[acyl-carrier-protein] synthase III FabH1 | 4 | 1 | 1.63 | 0.14 |
| 1432 | Autoinducer synthesis protein LasI | 2 | 1 | 3.10 | 0.05 |
| Secreted factors | |||||
| 3478 | Rhamnosyltransferase chain B RhlB | 4 | 1 | −1.57 | 0.15 |
| 1899 or 4210* | Probable phenazine biosynthesis protein PhzA | 2 | 2 | −2.69 | 0.00 |
| 1900 | Probable phenazine biosynthesis protein PhzB | 8 | 2 | −1.70 | 0.10 |
| 1903 or 4214* | Phenazine biosynthesis protein PhzE | 1 | 1 | −2.70 | na |
| 1904 or 4215* | Probable phenazine biosynthesis protein PhzF | 2 | 1 | −1.85 | 0.01 |
| 4211 | Probable phenazine biosynthesis protein PhzB | 2 | 1 | −1.93 | 0.07 |
| 4224 | Hypothetical protein PchG | 21 | 2 | −5.23 | 0.25 |
| 4225 | Pyochelin synthetase PchE | 4 | 3 | −1.97 | 0.14 |
| 4226 | Dihydroaeruginoic acid synthetase PchE | 15 | 3 | −4.23 | 0.19 |
| 4228 | Pyochelin biosynthesis protein PchD | 30 | 5 | −4.07 | 0.19 |
| 4230 | Salicylate biosynthesis protein PchB | 17 | 2 | −4.42 | 0.36 |
| 4231 | Salicylate biosynthesis isochorismate synthase PchA | 1 | 1 | −2.21 | na |
n, number of independent identifications and quantification events for each protein; u-ICAT, number of unique peptide sequences; fold abundance, average ratios of all quantified peptides for each protein representing fold increase in protein abundance during growth of P. aeruginosa in 8 μM Mg2+; na, not applicable. Positive values represent increased relative abundance on growth in low magnesium; negative values represent decreased relative abundance.
*
Identified peptides are identical in both proteins.