Table 2.
Differential P. aeruginosa protein fractionation on growth in low magnesium
| Gene no. | Protein | Membrane protein quantification
|
Whole cell protein quantification
|
M/WC abundance ratio | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| n | u-ICAT | Fold abundance | SD | n | u-ICAT | Fold abundance | SD | |||
| Energy metabolism | ||||||||||
| 1583 | Succinate dehydrogenase (A subunit) SdhA | 3 | 2 | 2.24 | 0.40 | 25 | 4 | 1.36 | 0.22 | 1.65 |
| 1584 | Succinate dehydrogenase (B subunit) SdhB | 7 | 3 | 2.20 | 0.28 | 2 | 1 | −1.14 | 0.09 | 2.50 |
| 1585 | 2-Oxoglutarate dehydrogenase (E1 subunit) SucA | 10 | 3 | 2.97 | 0.59 | 9 | 1 | 1.04 | 0.03 | 2.90 |
| 1770 | Phosphoenolpyruvate synthase PpsA | 6 | 1 | 3.41 | 1.07 | 40 | 3 | 1.12 | 0.23 | 3.04 |
| 5300 | Cytochrome c5 | 5 | 1 | 2.18 | 0.17 | 5 | 1 | 1.43 | 0.11 | 1.52 |
| 5554 | ATP synthase beta chain | 4 | 1 | 1.74 | 0.18 | 45 | 1 | 1.14 | 0.13 | 1.53 |
| 5555 | ATP synthase gamma chain | 2 | 1 | 1.98 | 0.03 | 11 | 1 | 1.33 | 0.08 | 1.48 |
| 5556 | ATP synthase alpha chain | 4 | 1 | 2.00 | 0.32 | 45 | 1 | 1.47 | 0.12 | 1.36 |
| 5557 | ATP synthase delta chain | 5 | 1 | 2.15 | 0.08 | 5 | 1 | 1.17 | 0.17 | 1.84 |
| Translation | ||||||||||
| 3656 | 30S ribosomal protein S2 | 6 | 1 | 2.01 | 0.32 | 15 | 1 | 1.27 | 0.05 | 1.58 |
| 4239 | 30S ribosomal protein S4 | 9 | 2 | 2.19 | 0.22 | 70 | 6 | 1.43 | 0.28 | 1.53 |
| 4241 | 30S ribosomal protein S13 | 5 | 1 | 2.42 | 0.05 | 74 | 3 | 1.32 | 0.23 | 1.83 |
| 4246 | 30S ribosomal protein S5 | 6 | 1 | 2.18 | 0.24 | 60 | 1 | 1.46 | 0.16 | 1.50 |
| 2071 | Elongation factor G | 2 | 1 | 2.17 | 0.30 | 11 | 1 | 1.29 | 0.12 | 1.68 |
| 4266 | Elongation factor G | 9 | 1 | 2.41 | 0.22 | 21 | 2 | 1.21 | 0.11 | 1.99 |
| Other | ||||||||||
| 4385 | GroEL protein | 3 | 1 | −1.16 | 0.02 | 3 | 1 | −3.03 | 0.64 | 2.61 |
| 2991 | Soluble pyridine nucleotide transhydrogenase | 1 | 1 | 1.64 | na | 3 | 1 | 1.00 | 0.05 | 1.64 |
| 4670 | Ribose-phosphate pyrophosphokinase | 23 | 2 | 2.13 | 0.22 | 10 | 2 | 1.31 | 0.12 | 1.63 |
| 3068 | Conserved hypothetical protein | 1 | 1 | 2.13 | na | 6 | 1 | 1.01 | 0.05 | 2.11 |
| 3263 | Conserved hypothetical protein | 3 | 1 | 1.66 | 0.10 | 1 | 1 | 1.06 | na | 1.56 |
| 2069 | Probable carbamoyl transferase | 3 | 1 | −9.10 | 0.02 | 2 | 2 | −1.06 | 0.11 | −8.58 |
n, number of independent identifications and quantification events for each protein; u-ICAT, number of unique peptide sequences; fold abundance, average ratios of all quantified peptides for each protein representing fold increase in protein abundance during growth of P. aeruginosa in 8 μM Mg2+; M/WC abundance ratio, ratio of relative protein abundances determined on ICAT analysis of membrane fraction and ICAT analysis of whole cell protein. Positive values represent increased relative abundance on growth in low magnesium; negative values represent decreased relative abundance.