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Localization of positions of cleavage of 16S rRNA in t30S particles by hydroxyl radicals generated from Fe(II) tethered to position 922. The 3′-domain transcripts have either GMP (lanes 1 and 3) or BABE–GMPS (lanes 2 and 4) at their 5′ termini. Lanes 1 and 2 are control lanes, in which ascorbate and H2O2 were omitted from the probing reaction. Locations of the cleavage positions are indicated by vertical bars. A and G are sequencing lanes.
