Figure 3.

Method for projecting image stacks to measure spindle angle. Stacks of 1-μm-thick optical images were projected into 3D images (A) by using Zeiss lsm software. These reconstructions were then rotated around the y axis until the edge of the metaphase plate (green) was resolved (B). This rotation brings the spindle axis parallel to the computer screen. Finally, a line through the spindle axis (dashed black line) was projected to another (red) line drawn parallel to the ventricular surface and the angle between the two lines was measured (C).