Figure 2.

Effect of med1, med2, and srb11 disruptions on GAL1 expression. (A) Single and double disrupted yeast strains as well as a wild-type control were grown to mid-log phase in the presence of 3% raffinose. Galactose then was added to a final concentration of 2%. The level of GAL1 expression was monitored at indicated timepoints after induction by using a GAL1-lacZ reporter gene as described (25). (B) GAL1-lacZ expression in the same strains when grown in 2% glucose (repressing conditions), 3% raffinose, or 3% glycerol-lactate (nonrepressing, noninducing conditions). The strains used were W303–1A, H707, H713, H715, H905, H909, and H922 (Table 1).