Figure 3.

Elution profiles of RNA polymerase II and mediator subunits during chromatography on hydroxyapatite. Proteins from the wild-type W303–1A (A) and med1 H707 (B) strains eluting in the 550 mM potassium acetate fraction from the DEAE-sephacel columns were applied to 10 ml HAP columns and were developed with a 100 ml-gradient of 0.01–0.2 M potassium phosphate. Fractions eluting from the columns were analyzed on a 10% SDS/PAGE gel and were immunoblotted with antibodies specific for different proteins indicated to the left. “L” represents proteins loaded to the HAP column, and “f.t” represents proteins present in the flow-through.