Table 2.
Ability of lexA fusion proteins to activate transcription
| Fusion protein | Wild-type | med1 | srb11 | med2 | Vector |
|---|---|---|---|---|---|
| lexA-Bicoid | 0.8 (0.1) | 0.9 (0.0) | 0.9 (0.0) | 0.9 (0.0) | CEN6 |
| lexA-Med1 (exp 1) | 1.5 (0.0) | 4.3 (0.1) | 432 (5.5) | 4.4 (0.3) | CEN6 |
| lexA-Med1 (exp 2) | 1.9 (0.1) | 3.7 (0.1) | 359 (4.5) | 3.1 (0.1) | CEN6 |
| lexA-Bicoid | 1.9 (0.5) | 1.3 (0.1) | 0.9 (0.1) | 1.8 (0.3) | 2 micron |
| lexA-Med1 | 526 (130) | 763 (122) | 441 (49) | 32 (3.5) | 2 micron |
| lexA-VP16 | 2030 (113) | 2477 (21) | 2360 (41) | 1628 (416) | CEN6 |
| lexA-Med12–40-VP16 | 1998 (142) | 2451 (43) | 1644 (102) | 2174 (35) | CEN6 |
Plasmids expressing different lexA fusion proteins (see Materials and Methods) were transformed into wild-type (W303-1A), med1 (H707), srb1 (H713), and med2 (H905) yeast strains together with the pSH18-34 reporter, which has several lexA binding sites in front of a CYC1-lacZ fusion gene. The values shown are β-galactosidase units with standard errors in parentheses. All cells were grown in synthetic 8% glucose media.