Figure 2. Changes in NF-κB sequence-specific binding activity in wounded IEC-6 cells in the presence and absence of bile salts. (A) Representative autoradiograms from control cells and cells 2 and 8 hours after wounding as described above. Nuclear extracts were prepared from cells after wounding. Electrophoretic mobility shift assay (EMSA) was performed using 10 υg nuclear protein and 0.035 pmol ³²P-end-labeled oligonucleotides containing a single NF-κB. Positions of the specifically bound DNA-protein binding complex are indicated. (B) Effect of the addition of 0.05 mmol/L TDCA to the medium of IEC-6 cell on sequence-specific NF-κB binding activity measured by EMSA. (C) Effect of 0.05 mmol/L TDCA and wounding on sequence-specific NF-κB binding activity measured by EMSA 6 hours after the addition of TDCA and wounding of the IEC-6 monolayer.