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. 1999 Jan 19;96(2):382–387. doi: 10.1073/pnas.96.2.382

Figure 1.

Figure 1

Cyclin E interacts with HPV E1. (a) Cdk-independent interaction of E1 and E1/E2 complexes with cyclin E in insect cells. Cells were infected with the indicated viruses and complexes were analyzed by SDS/PAGE and Coomassie staining after purification using GSH–Sepharose (lanes 1–10). Proteins were confirmed by immunoblotting (data not shown). In lanes 11–14, lysates from cells expressing E1, E2, or E1/E2 were mixed with cyclin E-containing lysates before purification. (b) Association of cyclin E/Cdk2 and E1 in COS-1 cells. Lysates from cells transfected with the indicated plasmids (Right) were subjected to immunoprecipitation and immunoblotting by using the indicated antibodies (Left and Center). Quantities of plasmids were: pMT2-E1*, 10 μg; pCMV-cyclin E, 1 μg; pCMV-Cdk2, 1 μg plus empty pMT2 to maintain DNA levels.