Survival of ES cells ipsilateral to the lesion at 60 days. Upper panel: Immunodetection
of GFP (A, DAB staining), NeuN (B, immunofluorescence), and
GFAP (C, immunofluorescence) in the ipsilateral dorsal horn. Small
white or black arrows indicate neurons. Big white or black arrow points
at collapsed dorsal horn. Lower panel: Double immunofluorescence
for GFP and MAP-2 (a neuronal marker). (A) GFP fluorescent cells. (B) MAP-2–positive
cells. (C) Merge of 2 images. Small white arrows
indicate neurons.