Figure 5.

Immunodetection of GRIK and GRIMP in Plant Tissues and Cultured Cells.

(A) Total protein extracts from young leaves (lanes 2 and 4), mature leaves (lanes 3 and 5), or infected mature leaves (lane 6) of Arabidopsis (lanes 2 and 3) or N. benthamiana (lanes 4 to 6) were separated by SDS-PAGE and detected using anti-GRIMP antibody. Recombinant GST-GRIMP (lane 1) was used as a control.

(B) Total protein extracts from Arabidopsis (lane 2) or N. benthamiana (lane 4) cultured cells were resolved by SDS-PAGE and detected using anti-GRIK antibody. Recombinant His-GRIK (lanes 1 and 3) was used as a control.