Table 1.
Characteristics of Tobacco BY-2 Cell Lines
| Percentage of Cells in Cell Cycle Phases
|
||||
|---|---|---|---|---|
| Cell | Doubling Time (h) | G1 | S | G2/M |
| GFP | 40.6 ± 4.3 | 50 (20.3) | 14 (5.7) | 36 (14.6) |
| CycD3;3-GFP | 36.6 ± 4.4 | 37 (13.5) | 27 (9.9) | 36 (13.2) |
| T191A-GFP | 41.4 ± 7.6 | 48 (19.9) | 14 (5.8) | 38 (15.7) |
| S296,300A-GFP | 29.9 ± 3.7 | 31 (9.3) | 32 (9.6) | 37 (11.1) |
The doubling time of tobacco BY-2 cells transformed with each construct were determined by measuring cell numbers. Data shown are means ± sd obtained from at least four sampling times with three independent samples for each time point. The percentage of cells in G1-, S-, and G2/M-phases was determined by LSC analysis. The data presented are a representative sample of many experiments. Mean times for each cell cycle phase were calculated assuming that all cells in the culture were cycling and are shown in hours.