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. 1999 Jan 19;96(2):394–399. doi: 10.1073/pnas.96.2.394

Figure 6.

Figure 6

Effect of various lipids on the activity of unphosphorylated MIHCK with and without Rac. (A) The activity of unphosphorylated MIHCK was assayed at 30°C in the absence (open bars) or presence of 50 μM (crossed bars) or 500 μM (black bars) phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidic acid (PA), linoleic acid (LA), oleic acid (OA), sphingosine (SPH), or ceramide (CER). The cross-hatched bar (PK) shows the activity of fully phosphorylated (maximally activated) MIHCK under the same conditions. (B) Activation of unphosphorylated MIHCK by Rac in the presence of various lipids. Activity was measured for 1 min at 20°C in the presence of phosphatidylserine (PS), phosphatidylinisitol (PI), phosphatidic acid (PA), or linoleic acid (LA) at the indicated concentrations in the absence (hatched bars) or presence (solid bars) of GST–Rac–GTP[γS]. The open bar (K) shows the activity of unphosphorylated MIHCK in the absence of lipid and Rac.