Figure 1.

Alignment of the Pore Region of Plant shaker-Like K⁺ Channels.

(A) Fifty-one amino acids from the end of the transmembrane region of S5 to the beginning of S6 are shown. The amino acids mutated in this study are indicated with arrows. Regions of interest are emphasized by boxes. Gray boxes indicate the predicted S5, pore, and S6 regions.

(B) Structural model of the KcsA channel depicting the equivalent positions of His-228 and Ser-271 in AKT3. An alignment of AKT3 with KcsA revealed that His-228 would reside in the descending loop and Ser-271 would reside in the ascending loop of the AKT3 channel.