Figure 1.
Seasonal Expression of PtABI3, PtABI1b, and PtACT in Poplar.
Expression was analyzed in seeds (7 and 27 days after pollination), in buds (from August until March), at bud break (BB), in young leaves (L; harvested in April), and in xylem (X). Fragments of the genes were amplified by reverse transcriptase–mediated (RT)–PCR using gene-specific primers, hybridized with 32P-labeled probes of the respective genes, and visualized through autoradiography. Actin fragments could be amplified from all samples, indicating that cDNA was generated successfully, although the amount of actin transcripts varied and was particularly low in January. This experiment was repeated with similar results with independent bud material.