. 2002 Aug;14(8):1903–1917. doi: 10.1105/tpc.002238

Table 4.

Results of Methylation and Expression Tests of Predicted Genes in the Barley Mla Region

						Methylation Test^b
Gene Abbreviation (in Figure 5)	Gene	Primer Pair	Primer Position^a	Annealing Temperature	Size (bp)	Morex	Steptoe	C.I. 16151	C.I. 16155	Transcription
g1	711N16.16	CGTAGATAGACAACGGAATCGAGG-GGGCGGGGTTCAACATATTG	CDS	58	271	−	+	−	−	+
g2	711N16.15	CGGACGAACAGTTGGTGTGCACCA-GGAGGAAGGAACACGC	CDS	62	215	−	−	−	−	+
g3	711N16.14	CGATCACAGTCACCGAGCAATCTG-AAGATAGCCAACGCCCTG	CDS	58	138	++	++	++	++	−
Bpm	Bpm^b	ATAAGCCGCCTTGCATAATACGAA-CTACCACTTCACACCTTGCAGG	Intron	55	625	+	+	−	+	+
g5a&b	Bmt^b	TTCTAGCAAGCATCTAAAGGTTTCG-ACATCTGTTAGTCGGGCAAGAGAC	3′ UTR	54	576	+	+	+	+	−
Bdi	Bdi	AGGGCATCTTCAATGGTCCTATGC-GGAGCTTGTGCGTGAACC	Predicted CDS	51	136	++	++	++	++	+
g8	711N16.9	CGACATTGTCCTGCTCTGTTGCCG-GTTTGAATTGGACGAGCTTG	CDS	58	134	++	++	NP	++	+
g9	711N16.8	TGTTGACTCCTTGATTCCATCCACC-TGCTAGATAAAGGCATCGTACCTG	CDS	57	582	++	++	++	++	+
3a&3b	RGH3a/b	GGTGTGTGATTTCGATGCCCAGGA-GCCTGCACCGTCT	CDS	54	146	+	+	+	NP	+
g11a&b	80H14.15	ACCCTGCTGCCGATCTACTGATAG-ATCGTTCCTCTTCCTCGTCGTC	CDS	59	157	++	++	++	++	−
g12a&b	80H14.14^c	GAGCACACTGGATTGTTGAAATGG-CACCCATGCCTTCCATAGTAGC	CDS	58	374	++	++	++	++	−
2a&b	RGH2a/b	ACCCTCGCCAGACAAGTTTACCGA-AAAGCAGCTTATGCACGTCG	CDS	57	165	++	NP	++	NP	+
1e&f	RGH1e/f	CATGGTTAGCCTGATCTCCAAGCT-GGAATTGCTCCGTGATTTCC	CDS	57	372	++	NP	++	NP	+
g15a&b	80H14.11	TCACACCATACGCCGCAACAGGTG-CTCACCGCAACGTC	CDS+Intron	64	378	++	++	++	++	−
g16a&b	80H14.10	CCTTTGTCGTTAGGATCGCATTCGT-TGGTAGGGTTATTGTTCATGCAC	CDS	56	131	++	++	++	++	+
1bcd	RGH1bcd	GAGTAATTGTCGCCGTTTGTCCGC-AACACACCAACTAGAGGAAACAC	CDS	54	434	−	+	++	++	+
g25	80H14.8^b	TCGGTGACGGTGTGACAATTCCTT-GATCTTCCCCTTGCTTGC	Intron+CDS +3′ UTR	58	493	−	NP	−	−	+
CI2f	CI2f	GGACCTGGGCAATGTTGTCGCAAA-GGAAGCAAGCGTAACAAGG	5′ UTR+CDS	58	264	−	−	−	+	+
CI2e	CI2e	GCAACCCAACTAGCCAACGTGGC-CAAGGAGATCATTCTCAAGGAC	CDS+3′ UTR	59	150	+	+	+	++	+
1a	RGH1a	TTGGGAAAAATAGCAGCCTGCATG-CAAGGGGTCTCTCATTATCC	CDS	56	572	−	++	−	−	−
CI2d	CI2d	GCTGACCCAAAAGCACCCGCTAG-CCGATGTGAGGAGTGGTC	CDS	59	216	−	−	−	−	+

The position of the primer pair is designated as follows: CDS, coding sequence; UTR, untranslated region.

Methylation test: +, only HpaII sensitive, internal C methylated; ++, both HpaII and MspI sensitive, external or both Cs methylated; −, not methylated; NP, no PCR product amplified from unrestricted genomic DNA.

These primers were used only for methylation tests. For RT-PCR, primers 5′-GAGCACACTGGATTGTTGAAATGG-3′ and 5′-GGAGCTTGATAGGTGTTGGCAAG-3′ were used at an annealing temperature of 55°C to amplify a 169-bp 80H14.14 product, primers 5′-AGAAAAGTTCCAGAAGATG-3′ and 5′-AAAAGACTATTTCGATTCC-3′ were used at an annealing temperature of 44°C to amplify a 117-bp Bmt product, primers 5′-ACTTCTTCAGTGTGTTCAGGTGAG- C-3′ and 5′-GCAAGCCACATGAGAGAACTGC-3′ were used at an annealing temperature of 55°C to amplify a 387-bp Bpm product, and primers 5′-GCTACTGGAGCCATCACATGAAG-3′ and 5′-CTTGATCTTCCCCTTGCTTGC-3′ were used at an annealing temperature of 58°C to amplify a 209-bp 80H14.8 product.