Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Aug;14(8):1919–1935. doi: 10.1105/tpc.003368

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, American Society of Plant Biologists

PMC Copyright notice

Figure 4. — Coimmunoprecipitation Assays.

(A) The Flag-tagged COI1 forms an SCF complex in Arabidopsis. Protein extracts from coi1-1 (lane 1) and transgenic coi1-1 expressing Flag-tagged COI1 (coi1-1::Flag-COI1; lane 2) were immunoprecipitated with α-Flag antibody (lanes 3 and 4).

(B) ASK2 associates physically with COI1 but not with ASK1 in planta. Protein extracts from a control wild-type plant (lane 5) and a transgenic plant expressing the Myc-tagged ASK2 (WT::Myc-ASK2; lane 6) were immunoprecipitated with α-Myc antibody (lanes 7 and 8).

(C) COI1_E22A abrogates SCF^COI1. Protein extracts from coi1-1 plants transgenic for vector (coi1-1::Myc; lane 9), Myc-tagged COI1_E22A (coi1-1:: Myc-COI1_E22A; lane 10), or Myc-tagged COI1 (coi1-1::Myc-COI1; lane 11) were immunoprecipitated with α-Myc antibody (lanes 12 to 14).

The resulting immunoprecipitates were resolved by SDS-PAGE and detected with the indicated antibodies. Molecular mass standards are given in kD. Arrows in (A) and (C) indicate the modified version of AtCUL1. The asterisk in (B) indicates the position of a nonspecific immunoreacting band. Hc, immunoglobulin heavy chain; IP, immunoprecipitate; Lc, immunoglobulin light chain; WT, wild type.