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The Abundance of the Modified AtCUL1 of SCFCOI1 Was Reduced in the axr1-3 Mutant.
Protein extracts from axr1-3 (lane 1), axr1-3 expressing Flag-tagged COI1 (axr1-3::Flag-COI1) (lane 2), and wild-type AXR1 plants expressing Flag-tagged COI1 (AXR1::Flag-COI1) (lane 3) were immunoprecipitated with α-Flag antibody (lanes 4 to 6). The α-Flag immune complexes were probed with α-COI1, α-AtCUL1, or α-ASK1 antisera. The arrow indicates the modified version of AtCUL1.
