FIG. 1.

PFGE patterns of genomic DNA of the wild serovar Typhimurium LT7 strain stored at −70°C cleaved with the endonucleases XbaI, AvrII, I-CeuI, and SpeI. Lanes: 1, XbaI cleavage of LT7 (3 small fragments—W [6.5 kb], X [6.4 kb], and Y [1 kb]—had run out of the gel); 2, I-CeuI cleavage of LT7; 3 and 4, AvrII cleavages of LT2 and LT7, respectively, for a comparison; two small fragments of LT7 had run out of the gel, including L (4 kb) and M (2 kb); 5, SpeI cleavage of LT7 (12 small fragments, X through II, ranging from 49 to 7 kb, had run out of the gel); 6, SpeI cleavage of LT7 serB::Tn10, showing the size shift of S112 with the Tn10 insertion (it is now 112 + 9, i.e., 121 kb).