FIG. 8.

Diverse genome structures among representative serovar Typhimurium LT7 mutants. (A) PFGE patterns of I-CeuI-cleaved genomic DNA of representative serovar Typhimurium LT7 mutants; (B) I-CeuI maps of these strains based on the data from panel A. In lane 3, the loss of the I-CeuI cleavage site in rrlD resulted in the disappearance of I-CeuI C; the disappearance of I-CeuI B and the appearance of I-CeuI B+C (1306 kb) are not obvious on this picture but have been confirmed in other experiments (data not shown). These maps, although constructed based initially on only I-CeuI data, were all confirmed by long-range PCR. The solid circle in I-CeuI C indicates the position of oriC.