TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli | ||
| DH5α | Recipient for cloning experiments | 17 |
| S17-1λpir | Recipient for suicide plasmids | 43 |
| BL21(DE3) | IPTG-inducible T7 RNA poly- merase | 47 |
| Yersinia | ||
| YPIII(pIB102) | wild-type, parental strain | 2 |
| YPIII(pIB69) | yscP-null strain | This study |
| YPIII(pIB75) | yscU-null strain | 25 |
| YPIII(pIB202) | yscF-null strain | This study |
| YPIII(pIB604) | yopB-null strain | 16 |
| YPIII(pIB82) | yopN-null strain | 39 |
| YPIII(pIB60469) | yopB yscP-double-null strain | This study |
| YPIII(pIB6975) | yscP yscU-double-null strain | This study |
| YPIII(pIB8769) | yopN yscP-double-null strain | This study |
| YPIII(pIB70169) | lcrG yscP-double-null strain | This study |
| YPIII(pIB2669) | lcrQ yscP-double-null strain | This study |
| YPIII(pIB62169) | yopD yscP-double-null strain | This study |
| Plasmid | ||
| pBAD33 | Contains PBAD promoter | 15 |
| pET-22b | T7 overexpression vector | Novagen |
| pDM4 | Suicide vector | 28 |
| pSL144 | ΔyscP in pDM4 | This study |
| pSL340 | ΔyopN in pDM4 | This study |
| pKK223-3 | Contains tac promoter | Amersham Phar- macia Biotech |
| pPE5 | yscU gene in pET-22b | This study |
| pPE33 | yscU gene in pKK223-3 | This study |
| pPE34 | yscP gene in pBAD33 | This study |
| pPE36 | yscU-A268F gene in pKK223-3 | This study |
| pPE37 | yscU-Y287G gene in pKK223-3 | This study |
| pPE38 | yscU-V292T gene in pKK223-3 | This study |
| pPE39 | yscU-Y317D gene in pKK223-3 | This study |
| pMMB66EH | Contains tac promoter | 14 |
| pJO33 | GST-YscF overexpression vector | This study |
| pGEX-5X-3 | Cloning vector | Amersham Phar- macia Biotech |
| pLS52 | ΔyscF in pDM4 | This study |
| pLS53 | YscF expression vector | This study |