TABLE 2.
Apparent dissociation constants (KD) from gel shiftsa
| Binding site | Mean apparent KD (nM) ± SD |
|---|---|
| F-F | 250.00 ± 18.0 |
| F-F-F | 36.10 ± 7.1 |
| F-F-F-F | 6.30 ± 0.6 |
| F-F-x-R | 0.95 ± 0.1 |
| F-F-x-R-R | 0.78 ± 0.2 |
| fepB | 8.60 ± 1.3 |
| entS | 3.01 ± 0.8 |
KD is the value at which 50% of the DNA is bound by Fur and is given as the Fur concentration at which this occurred.