TABLE 2.

Kinetic parameters for different OTCases

Organism	Max growth rate temp (°C)	Assay conditions (pH)	Assay temp (°C)	Km Apparent (mM) for:		kcat (s−1)	kcat/Km for:		Source or reference
				Ornithine	CP		Ornithine	CP
M. abyssi	4	Tris (9.0)a	30	45.0	0.9	690	15	766	This work
			25	22.49		750	33		This work
			20	8.0b	1.0b	694	87	694	This work
			15	5.67		546	97		This work
			10	3.34		382	114		This work
			5	1.78b	1.1b	235	132	214	This work
Saccharomyces cerevisiae	30	Tris (8.5)	30	0.9	0.2	354	393	1,770	13
E. coli W	37	Tris (8.0)	37	2.4b	0.2b	1,732	722	8,660	31
T. thermophilus	75	Tris (7.0)	55	0.1	0.1	74	740	740	45
			70			146c
P. furiosus	102	Tris (7.3) or PIPES (7.0)	55	0.13	0.13	35	269	269	33
			95			175c

^aThere were no significant differences in K_m values at pH 7.5 and 20°C.

^bReal K_m.

^cExtrapolated due to CP thermolability.