TABLE 3.
Half-lives of activities of psychrophilic, mesophilic, and thermophilic OTCases
| Organism | Temp (°C) | Half-lifea in the following buffer (pH):
|
||
|---|---|---|---|---|
| Potassium phosphate (7.0) | PIPES-NaOH (7.0) | Tris HCl (7.5) | ||
| M. abyssi | 50 | 60 | 14 | 9 |
| 55 | 40 | 4.5 | 3 | |
| 60 | 2 | |||
| E. coli | 68 | 43b | 5 | |
| P. furiosus | 100 | 65 | 60 | 40 |
Reported in minutes.
Value was determined with 10 mM potassium phosphate in 300 mM Tris HCl (pH 8.0).