FIG. 5.
(A) Expression of ywdL-lacZ during sporulation. Strain KB43 (ywdL-lacZ) was induced to sporulate by resuspension at time t0, and samples were removed for estimation of β-galactosidase specific activity (▪) and DPA content (⋄) as described in Materials and Methods. The DPA curve is used here as a marker of the progression through sporulation. (B) Expression of ywdL-lacZ in strains that lack one of the sporulation-specific RNA polymerase σ factors. Strains KB46 (sigF ywdL-lacZ) (○), KB45 (sigE ywdL-lacZ) (▴), KB47 (sigG ywdL-lacZ) (•), KB44 (sigK ywdL-lacZ) (▵), and KB43 (ywdL-lacZ) (▪) were induced to sporulate by resuspension, and the β-galactosidase specific activity was determined as described for panel A. The maximum β-galactosidase specific activity of strain PY79, which was induced to sporulate in parallel with the mutant strains, was found to be 4 Miller units.