TABLE 2.
Germination of spores of various strains with nutrients and Ca2+-DPA
| Genotype (strain) | Spore germination with:
|
|
|---|---|---|
| Nutrients (CFU/ml)a | Ca2+-DPAb | |
| Wild type (PS832) | 3.6 × 107 | + |
| ywdL5 (KB1) | 3.6 × 107 | − |
| ywdL16 (KB2) | 4.6 × 107 | − |
| ΔywdL (KB29) | 5.2 × 107 | − |
| ΔsleB (FB110) | 3.4 × 107 | + |
| ΔsleB ΔywdL (KB30) | 1.8 × 102 | − |
| ΔsleB ywdL5 (KB4) | 1.1 × 103 | − |
| ΔsleB ywdL16 (KB3) | 1.2 × 102 | − |
| ΔcwlJ ΔsleB (FB113) | 1.5 × 102 | − |
| ΔsleB ywdL-gfp (KB50) | 3 × 106 | − |
a
Heat treated spores were prepared at an OD600 of 1, and then serial dilutions of spores in water were spotted on LB agar plates and incubated at 30°C for 24 h and the CFU per milliliter were determined as described in Materials and Methods. Values shown are the averages from two independent experiments. The individual experimental values were within 45% of the average.
b
Spores at an OD600 of 1 were incubated in 60 mM Ca2+-DPA (pH 8.0) at 25°C for 1 h and then checked by phase-contrast microscopy for spore germination. + and −, 99% germinated and dormant spores, respectively.