TABLE 2.
Quorum-repressed genes
| Gene no.a | Description | Maximum changeb
|
||
|---|---|---|---|---|
|
lasI rhlI mutant
|
Wild type vs lasR rhlR mutant | |||
| 3OC12-HSL | C4-HSL + 3OC12-HSL | |||
| PA0165 | Hypothetical protein | −2.7 | −2.9 (2.0) | −4.8 (2.0) |
| PA0433 | Hypothetical protein | −6.8 | −20 (2.0) | −8.9 (1.4) |
| PA0434 | Hypothetical protein | −7.7 | −8.5 (2.0) | −5.6 (2.0) |
| PA0435 | Hypothetical protein | −9.4 | −26 (2.0) | −34 (2.0) |
| PA0485 | Conserved hypothetical proteinc | −1.7 | −3.4 (1.4) | −3.0 (3.0) |
| PA0887 | acsA, acetyl-coenzyme A synthetase | −3.3 | −4.2 (2.0) | −3.6 (3.0) |
| PA1559 | Hypothetical protein | −2.4 | −3.5 (2.0) | −3.2 (1.4) |
| PA2007 | maiA, maleylacetoacetate isomerase | −3.2 | −1.4 (4.0) | −3.2 (3.0) |
| PA2008 | fahA, fumarylacetoacetase | −3.7 | −1.5 (4.0) | −2.6 (3.0) |
| PA2009 | hmgA, homogentisate 1,2-dioxygenase | −4.0 | −1.5 (4.0) | −2.7 (3.0) |
| PA2250 | lpdV, lipoamide dehydrogenase-Val | −3.1 | −1.8 (4.0) | −2.6 (3.0) |
| PA2338 | Probable component of ABC maltose transporter | −5.0 | −3.2 (3.0) | −4.2 (3.0) |
| PA2339 | Probable maltose-mannitol transport protein | −1.9 | −6.8 (3.0) | −4.1 (3.0) |
| PA2340 | Probable maltose-mannitol transport protein | −3.4 | −2.0 (3.0) | −3.7 (3.0) |
| PA2341 | Probable component of ABC maltose transporter | −3.1 | −2.0 (3.0) | −4.2 (3.0) |
| PA2343 | mtlY, xylulose kinase | −1.7 | −4.0 (3.0) | −3.2 (4.0) |
| PA3038 | Probable porin | −2.3 | −3.5 (2.0) | −4.4 (3.0) |
| PA3174 | Probable transcriptional regulator | −2.1 | −3.5 (4.0) | −6.5 (3.0) |
| PA3205 | Hypothetical protein | −1.3 | −3.1 (4.0) | −3.1 (4.0) |
| PA3233 | Hypothetical protein | −2.2 | −2.7 (3.0) | −5.1 (3.0) |
| PA3234 | Probable sodium-solute symporter | −4.5 | −3.4 (2.0) | −7.0 (3.0) |
| PA3235 | Conserved hypothetical protein | −3.9 | −4.2 (3.0) | −6.6 (3.0) |
| PA3281 | Hypothetical protein | −5.7 | −6.4 (1.4) | −25 (1.4) |
| PA3282 | Hypothetical protein | −8.5 | −8.8 (1.4) | −21 (1.4) |
| PA3283 | Conserved hypothetical protein | −9.0 | −8.8 (1.4) | −28 (1.4) |
| PA3284 | Hypothetical protein | −7.1 | −10 (2.0) | −24 (1.4) |
| PA3364 | amiC, aliphatic amidase expression-regulating protein | −2.7 | −1.8 (4.0) | −2.7 (1.4) |
| PA3365 | Probable chaperone | −3.0 | −1.7 (4.0) | −4.0 (1.4) |
| PA3575 | Hypothetical protein | −1.6 | −2.7 (1.4) | −3.3 (2.0) |
| PA3790 | oprC, outer membrane protein OprC | −2.7 | −3.7 (2.0) | −4.6 (2.0) |
| PA4359 | Conserved hypothetical protein | −1.4 | −2.7 (2.0) | −2.8 (1.4) |
| PA4371 | Hypothetical protein | −1.9 | −4.1 (2.0) | −2.8 (1.4) |
| PA4442 | cysN, ATP sulfurylase GTP-binding subunit | −2.8 | −3.4 (3.0) | −7.6 (2.0) |
| PA4443 | cysD, ATP sulfurylase small subunit | −3.1 | −3.4 (3.0) | −6.5 (2.0) |
| PA4691 | Hypothetical protein | −2.5 | −2.8 (2.0) | −2.9 (2.0) |
| PA4692 | Conserved hypothetical protein | −3.8 | −3.4 (2.0) | −5.0 (1.4) |
| PA4770 | IldP, l-lactate permease | −1.8 | −3.7 (2.0) | −5.0 (2.0) |
| PA5168 | Probable dicarboxylate transporter | −2.7 | −1.9 (4.0) | −5.8 (2.0) |
a
Gene number from the Pseudomonas genome project (http://www.pseudomonas.com).
b
Maximum changes in gene expression (rounded to two significant figures) in the signal generation mutant in the presence of the signal(s) indicated compared with the absence of signal and in the wild-type P. aeruginosa strain compared with the receptor mutant. The values in parentheses are the OD600 at which the earliest change of ≥2.5-fold was observed (for the signal generation mutant both time courses were considered).
c
There is a las-rhl box-like sequence with an HI of ≥10 and <13.