Alignment of las boxes upstream of QS-regulated genes. Previously identified las boxes were aligned to form a consensus sequence (11, 29, 43). This sequence was used to search all upstream regions in front of the translational start site of all QS-regulated genes. The consensus sequence NHCTRNSNNDHNDKNNAGNB was derived from inspection, where H = C, T, or A; R = A or G; S = C or G; D = G, A, or T; K = G or T; B = G or T; and N = A, C, G, or T. Sequences that matched the consensus sequence were identified, and the position of the las box is shown in Table 1.