TABLE 5.
QS-regulated transcripts detected at the early stationary phase in PAO1 grown under various nutrient and oxygen conditionsa
| ORF or intergenic region | Gene name | Presence inb:
|
Protein descriptionc | ||
|---|---|---|---|---|---|
| NY, aerobic | NY + KNO3 aerobic | NY + KNO3, anaerobic | |||
| PA0007 | + | − | − | Hypothetical protein | |
| PA0040 | + | − | + | Conserved hypothetical protein | |
| PA0103 | + | − | − | Probable sulfate transporter | |
| PA0105 | coxB | + | + | − | Cytochrome c oxidase subunit II |
| PA0107 | − | − | − | Conserved hypothetical protein | |
| PA0108 | coxIII | + | − | − | Cytochrome c oxidase subunit III |
| PA0111 | + | − | − | Hypothetical protein | |
| PA0144 | − | − | − | Hypothetical protein | |
| PA0196 | pntB | + | − | + | Pyridine nucleotide transhydrogenase beta subunit |
| PA0228 | pcaF | − | − | − | Beta-ketoadipyl-coenzyme A thiolase PcaF |
| PA0239 | + | − | − | Hypothetical protein | |
| PA0244 | + | − | − | Hypothetical protein | |
| PA0365 | + | + | − | Hypothetical protein | |
| PA0524 | norB | − | + | + | Nitric oxide reductase subunit B |
| PA0996 | + | + | − | Probable coenzyme A ligase | |
| PA0997 | + | + | − | Hypothetical protein | |
| PA0998 | + | + | − | Hypothetical protein | |
| PA1063 | + | − | + | Hypothetical protein | |
| PA1212 | + | − | − | Probable MFS transporter | |
| PA1214 | + | − | − | Hypothetical protein | |
| PA1217 | + | − | − | Probable 2-isopropylmalate synthase | |
| PA1221 | + | − | + | Hypothetical protein | |
| PA1318 | cyoB | + | − | − | Cytochrome o ubiquinol oxidase subunit 1 |
| PA1327 | + | + | − | Probable protease | |
| PA1356 | − | − | − | Hypothetical protein | |
| PA1608 | + | + | − | Probable chemotaxis transducer | |
| PA1679 | − | + | + | Hypothetical protein | |
| PA1694 | pscQ | + | − | − | Translocation protein in type III secretion |
| PA1771 | + | − | − | Probable esterase/lipase | |
| PA1869 | + | + | − | Probable acyl carrier protein | |
| PA1875 | + | − | − | Hypothetical protein | |
| PA1877 | − | − | − | Probable secretion protein | |
| PA1892 | + | + | − | Hypothetical protein | |
| PA1893 | + | − | − | Hypothetical protein | |
| PA1894 | + | + | − | Hypothetical protein | |
| PA1895 | + | + | − | Hypothetical protein | |
| PA1896 | − | − | − | Hypothetical protein | |
| PA1897 | + | + | − | Hypothetical protein | |
| PA1898 | + | + | − | Probable transcriptional regulator | |
| PA1901 | + | + | − | Phenazine biosynthesis protein PhzC | |
| PA1902 | + | − | − | Phenazine biosynthesis protein PhzD | |
| PA1903 | + | + | − | Phenazine biosynthesis protein PhzE | |
| PA1904 | + | + | − | Probable phenazine biosynthesis protein | |
| PA1927 | metE | − | − | + | 5-Methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase |
| PA1976 | + | − | − | Probable two-component sensor | |
| PA1978 | + | + | − | Probable transcriptional regulator | |
| PA1983 | exaB | − | − | − | Cytochrome c550 |
| PA1992 | − | − | − | Probable two-component sensor | |
| PA2068 | + | − | − | Probable MFS transporter | |
| PA2081 | + | − | − | Hypothetical protein | |
| PA2190 | + | + | − | Conserved hypothetical protein | |
| PA2194 | hcnB | + | + | − | Hydrogen cyanide synthase HcnB |
| PA2196 | + | + | − | Probable transcriptional regulator | |
| PA2238 | + | + | − | Hypothetical protein | |
| PA2260 | + | − | + | Hypothetical protein | |
| PA2261 | + | − | − | Probable 2-ketogluconate kinase | |
| PA2262 | + | − | − | Probable 2-ketogluconate transporter | |
| PA2266 | + | − | − | Probable cytochrome c precursor | |
| PA2300 | chiC | + | + | − | Chitinase |
| PA2303 | + | + | − | Hypothetical protein | |
| PA2331 | + | + | − | Hypothetical protein | |
| PA2369 | + | − | − | Hypothetical protein | |
| PA2414 | + | + | − | l-Sorbosone dehydrogenase | |
| PA2415 | + | + | − | Hypothetical protein | |
| PA2454 | + | − | + | Hypothetical protein | |
| PA2539 | + | − | − | Conserved hypothetical protein | |
| PA2540 | + | − | + | Conserved hypothetical protein | |
| PA2564 | + | − | − | Hypothetical protein | |
| PA2566 | + | − | − | Conserved hypothetical protein | |
| PA2570 | palL | + | + | − | PA-I galactophilic lectin |
| PA2571 | + | + | − | Probable two-component sensor | |
| PA2572 | + | + | − | Probable two-component response regulator | |
| PA2588 | + | + | − | Probable transcriptional regulator | |
| PA2699 | − | + | − | Hypothetical protein | |
| PA2763 | + | − | − | Hypothetical protein | |
| PA2862 | lipA | + | + | − | Lactonizing lipase precursor |
| PA2903 | cobJ | + | − | + | Precorrin-3 methylase CobJ |
| PA2939 | + | + | − | Probable aminopeptidase | |
| PA2974 | + | + | − | Probable hydrolase | |
| PA3242 | + | + | − | Probable lauroyl acyltransferase | |
| PA3311 | + | − | − | Conserved hypothetical protein | |
| PA3328 | + | + | − | Probable FAD-dependent monooxygenase | |
| PA3329 | + | + | − | Hypothetical protein | |
| PA3330 | + | + | − | Probable short-chain dehydrogenase | |
| PA3332 | + | + | − | Conserved hypothetical protein | |
| PA3333 | fabH2 | + | + | − | 3-Oxoacyl-(acyl carrier protein) synthase III |
| PA3334 | + | + | − | Probable acyl carrier protein | |
| PA3335 | + | + | − | Hypothetical protein | |
| PA3336 | + | + | − | Probable MFS transporter | |
| PA3391 | nosR | − | + | + | Regulatory protein NosR |
| PA3394 | nosF | − | + | + | NosF protein |
| PA3395 | nosY | − | + | + | NosY protein |
| PA3478 | rhlB | + | + | − | Rhamnosyltransferase chain B |
| PA3479 | rhlA | + | + | − | Rhamnosyltransferase chain A |
| PA3519 | + | + | − | Hypothetical protein | |
| PA3709 | − | − | − | Probable MFS transporter | |
| PA3719 | + | + | − | Hypothetical protein | |
| PA3870 | moaAI | − | + | + | Molybdopterin biosynthetic protein A1 |
| PA3877 | narKI | − | + | + | Nitrite extrusion protein 1 |
| PA3914 | moeAI | − | + | + | Molybdenum cofactor biosynthetic protein A1 |
| PA4078 | + | + | − | Probable nonribosomal peptide synthetase | |
| PA4142 | + | − | − | Probable secretion protein | |
| PA4175 | + | − | − | Probable endoproteinase Arg-C precursor | |
| PA4209 | + | + | − | Probable O-methyltransferase | |
| PA4211 | + | + | − | Probable phenazine biosynthesis protein | |
| PA4293 | + | − | − | Probable two-component sensor | |
| PA4297 | − | − | − | Hypothetical protein | |
| PA4298 | + | + | − | Hypothetical protein | |
| PA4300 | + | − | + | Hypothetical protein | |
| PA4302 | − | − | − | Probable type II secretion system protein | |
| PA4303 | + | + | − | Hypothetical protein | |
| PA4304 | + | − | − | Probable type II secretion system protein | |
| PA4305 | + | − | − | Hypothetical protein | |
| PA4307 | pctC | + | − | − | Chemotactic transducer PctC |
| PA4384 | + | − | − | Hypothetical protein | |
| PA4498 | + | − | + | Probable metallopeptidase | |
| PA4648 | + | − | − | Hypothetical protein | |
| PA4649 | + | − | − | Hypothetical protein | |
| PA4651 | + | + | − | Probable pilus assembly chaperone | |
| PA4681 | + | − | + | Hypothetical protein | |
| PA4908 | + | + | − | Hypothetical protein | |
| PA4910 | + | + | − | Probable ATP-binding component of ABC transporter | |
| PA4911 | − | − | − | Probable permease of ABC branched-chain amino acid transporter | |
| PA4912 | + | − | − | Probable permease of ABC branched-chain amino acid transporter | |
| PA4979 | − | − | − | Probable acyl-coenzyme A dehydrogenase | |
| PA5059 | + | + | − | Probable transcriptional regulator | |
| PA5097 | − | − | − | Probable amino acid permease | |
| PA5181 | + | − | + | Probable oxidoreductase | |
| PA5375 | betTI | + | − | − | Choline transporter BetT |
| PA5411 | + | − | + | Probable ferredoxin | |
| PA5543 | + | − | + | Hypothetical protein | |
| ig 1427453-1428080 | + | − | − | Intergenic region between PA1372 and PA1373, 1427453-1428080, (+) strand | |
| ig 4713795-4713098 | + | + | − | Intergenic region between PA4280 and PA4281, 4713098-4713795, (−) strand | |
| ig 5820909-5820113 | + | − | + | Intergenic region between PA5321 and PA5322, 5820113-5820909, (−) strand | |
a
Genes are identified by ORF designation, gene name, and protein description (http://www.pseudomonas.com).
b
The presence of each transcript was analyzed by using MAS (version 5.0) and DMT (version 3.0) software for duplicate samples. Transcripts identified as absent on at least two of three chips were considered absent. +, present; −, absent.
c
FAD, flavin adenine dinucleotide.