FIG. 3.

Analysis of the methylation specificity of M.EcoT38I (ecoT38IM). (A) Structure of pUCEV, in which the 2.2-kb EcoRV fragment carrying the M.EcoT38I gene was cloned into the HincII site of pUC118. (B) Resistance to R.BanII of M.EcoT38I-modified DNA. pUCEV and pUC118 were incubated with R.BanII. The products were separated by 1% agarose gel electrophoresis. Lane 1, pUCEV; lane 2, pUCEV plus R.BanII; lane 3, pUC118; lane 4, pUC118 plus R.BanII. λ HindΙΙΙ digests were used for molecular size calibration (lane M).