FIG. 4.
Effect of C.EcoT38I on the promoter activities of the R.EcoT38I and M.EcoT38I genes. The segments from 1,158 to 1,239 bp and from 1,107 to 1,290 bp shown in Fig. 1 were cloned upstream of a promoterless AR1 gene in vector pMCLTerAR. Promoter activities associated with various DNA fragments were measured for both orientations. Each clone was assayed for AR1 activity in the absence (open bars) and presence (closed bars) of C.EcoT38I (graph). R, M, and C represent R.EcoT38I, M.EcoT38I, and C.EcoT38I, respectively.