TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| MG1655 | K-12 reference strain | 3 |
| MS428 | MG1655 fim | 16 |
| MS528 | MG1655 fim flu | 16 |
| MGJ1 | MG1655 oxyR | This study |
| BD1428 | Phase-variable Ag43 expression | 7 |
| BD1511 | Ag43 phase-off variant of BD1428 | 7 |
| BD1512 | Ag43 phase-on variant of BD1428 | 7 |
| Plasmids | ||
| pBAD/myc-HisA | Arabinose-inducible promoter | 8 |
| pMGJ1 | oxyR gene in pBAD/myc-HisA | This study |
| pMAS141 | oxyRRed,C199S gene in pBAD/myc-HisA | This study |
| pMAS142 | oxyROx,H198R gene in pBAD/myc-HisA | This study |
| pHHA147 | flu gene downstream of tet promoter in pBR322 | 11 |