TABLE 1.
Strains and vectors used
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| PAK | Wild type; piliated; twitching | 7 |
| PAK-NP | Tetr cassette inserted into pilA; nonpiliated; nontwitching | 19 |
| R364 | Tn5-B21 insertion in pilT; hyperpiliated; nontwitching | 21 |
| S34 | Tn5-B21 insertion in pilU; hyperpiliated; nontwitching | 22 |
| R364+ | R364 complemented with pUCP20Gm-pilT; twitching | This study |
| S34+ | S34 complemented with pUCP20Gm-pilU; twitching | This study |
| Plasmids | ||
| pUCP20 | Shuttle vector encoding beta-lactam resistance | 20 |
| pUCGm | Source of gentamicin resistance cassette | 14 |
| pUCP20Gm | pUCP20 with SmaI-flanked Gm cassette inserted into unique ScaI site within bla | This study |
| pUCP20Gm-pilT | pUCP20Gm with pilT cloned into BamHI-HindIII in MCS | This study |
| pUCP20Gm-pilU | pUCP20Gm with pilU cloned into HindIII in MCS | This study |