FIG. 1.
Deletions of the IIMtl complex of E. coli K-12. The locations of domains A, B, and C of IIMtl are indicated together with the locations of the essential residues E218, H256, C384, and H554 and the locations of the approximate borders between the domains. The longest deletions in the four deletion classes isolated previously are L611 to K637 in class 1, Q520 to K637 in class 2, M393 to K637 in class 3, and L161 to K637 in class 4. Representative deletions for each class are Δ69 (ΔL632-K637) and Δ65 (ΔE618-K637) for class 1, Δ130 (ΔV532-K637) for class 2, Δ137 (ΔE511-K637) for class 3, and ΔSnaBI (ΔV377-K637) and Δ21 (ΔE346-K637) for class 4. In K. pneumoniae, ΔKp (ΔG361-K635) was used as a representative of class 4 and corresponds to the allele present in pSOL110 (Table 1).