TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Origin and/or relevant properties | Reference or source |
|---|---|---|
| E. coli K-12 strains | ||
| JWL181 | mtlAp,o50(Con) gutA50 gatA50 | 17 |
| JWL191 | ptsI191 | 17 |
| LGS322 | JWL181 ΔmtlA55 mtlD+ Δ(gut-rec)63 Atls | 8 |
| LGS323 | LGS322 Atlr Crr− | 10 |
| LGS324 | LGS322 Atlr | This study |
| LGS31 | JWL181 ΔmtlA55 mtlD+gutA50 | 8 |
| LTK31-2 | LGS31 Δ(ptsHI crr)::kan/F′DalD/pUR404 | This study |
| STL136 | lac-85 malA1 mel-2 rpsL Δ(ptsHIcrr)::kan/F′DalD | This study |
| Other strains | ||
| 1033-5P14 | K. pneumoniae | 9 |
| M5a1 | K. oxytoca | 25 |
| Plasmids | ||
| pGJ9 | mtlA+E. coli (637 amino acids) | 8 |
| pGJ9Δ137 | mtlAΔ137 (deleted after bp 1530, amino acid 510) | 8 |
| pGJ9ΔSnaBI | mtlAΔSnaBI (deleted after bp 1128, amino acid 376) | 8 |
| pDW1 | mtlA+ (bp 1 to 1128 deleted, amino acids 1 to 376) | 50 |
| pSOL300 | pHEX3 mtlA+K. pneumoniae (635 amino acids) | 28 |
| pSOL110 | pHEX3 ΔmtlA′ (deleted after bp 929, amino acid 361) | 28 |
| pGHL3 | pACYC 177 dalD+K+T+K. oxytoca | G. Heilenmann |
| pASL14 | pUC18 dalD+ K′K. oxytoca (2.7-kb HindIII-EcoRI fragment) | This study |
| F′DalD | F′laclacI proA+B+::TnLSD101 dalD+K′K. oxytocaSpr | This study |
| pTIM101 | pIC-19H::TnLSD | 51 |
| pLSD101 | pTIM101 Apr::TnLSD101 (dalD+K′ spc) | This study |
| pUR404 | scr+ ScrR− Tcr | 1 |