TABLE 2.
Properties of the selection strains
| Strain | Plasmid | Growth ona:
|
IIMtl phosphorylation activity (nmol/min/mg of total membrane protein)b | MtlD activity (nmol/min/mg of total protein)b | DalT activity (nmol/min/mg of total protein)b | DalD activity (nmol/min/mg of total protein)b | |
|---|---|---|---|---|---|---|---|
| Mtl | Atl | ||||||
| JWL181 mtl+gutA50 | None | 3+ | s | 9.8 | 2.20 | ≤0.1 | ≤0.01 |
| pASL14 | 3+ | 2+ | NT | NT | ≤0.1 | 1.20 | |
| LGS322 ΔmtlA55 Δ(gut-rec)63 Atls | None | − | s | ≤0.1 | 0.73 | ≤0.1 | ≤0.01 |
| pGHL3 | 2+ | 3+ | ≤0.1 | NT | 10.0 | 1.19 | |
| pASL14 | − | 2+ | ≤0.1 | NT | ≤0.1 | 1.18 | |
| F′DalD | − | + | ≤0.1 | NT | ≤0.1 | NT | |
| LGS323 ΔmtlA55 Δ(gut-rec)63 Crr− Atlr | None | − | − | ≤0.1 | 0.75 | ≤0.1 | ≤0.01 |
| pGHL3 | 2+ | 3+ | ≤0.1 | NT | 9.5 | 1.18 | |
| pASL14 | − | − | ≤0.1 | NT | ≤0.1 | 1.21 | |
| F′DalD | − | − | ≤0.1 | NT | ≤0.1 | NT | |
| JWL191 ptsI191 | None | − | − | ≤0.1 | 1.52 | ≤0.1 | NT |
| pGHL3 | 2+ | 3+ | ≤0.1 | NT | 3.5 | 0.45 | |
| pASL14 | − | 3+ | ≤0.1 | NT | ≤0.1 | 0.40 | |
a
Growth on d-mannitol (Mtl) and d-arabinitol (Atl) was tested on MacConkey indicator plates containing 1% carbohydrate. −, white colonies; +, pink colonies; 2+ or 3+, purple or deep purple colonies; s, sensitive.
b
Cells in the exponential growth phase on minimal glycerol (0.2%, vol/vol) medium were harvested and tested as described in Materials and Methods. The IIMtl phosphorylation activity was tested with 30 μM d-mannitol, the d-mannitol-1-phosphate dehydrogenase (MtlD) activity was tested with 2 mM d-mannitol, the d-arabinitol transporter (DalT) activity was tested with 500 μM d-[14C]mannitol, and the d-arabinitol dehydrogenase (DalD) activity was tested with 2 mM d-arabinitol. NT, not tested.