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Uptake of [14C]l-ascorbate by the ΔyjfR mutant as a function of l-ascorbate concentration (1 to 300 μM concentration range). The uptake experiment was conducted as described in Materials and Methods after anaerobic growth in 20 mM l-ascorbate plus 20 mM d-glucitol. The inset shows a double-reciprocal plot of the data.
