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SDS-PAGE of YwtD produced in E. coli BL21(DE3) harboring pYWTD and the purified YwtD protein. SDS-PAGE was done with a 12% polyacrylamide gel at pH 7.0 by the method of Laemmli (13). The gel was stained with Coomassie brilliant blue R-250. Lane 1, marker proteins; lane 2, cell extracts without IPTG induction; lane 3, cell extracts with 0.5 mM IPTG induction; lane 4, purified YwtD after nickel-nitrilotriacetic acid column chromatography.
